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Submitted: 27 January 2014 Modified: 10 May 2014

Herdin Record #: NCR-RITM-14012714181230

Genetic and phenotypic characterization of sylvatic dengue virus type 4 strains

Researchers

NameRole
1S Rossi Author
2F Nasar Author
3J Cardosa Author
4S Mayer Author

Publication Information

1.
Publication Type:
Journal
Publication Sub Type:
Announcements
Title:
Virology
Frequency:
Monthly
Publication Date:
February 2002
Volume:
423
Issue:
1
Page(s):
58-67
Publisher:
Elsevier

Abstract

Four serotypes of dengue virus (DENV 1-4) currently circulate between humans and domestic/ peridomestic Aedes mosquitoes, resulting in 100 million infections per year. All four serotypes emerged, independently, from sylvatic progenitors transmitted among non-human primates by
arboreal Aedes mosquitoes. This study investigated the genetic and phenotypic changes associatedwith emergence of human DENV-4 from its sylvatic ancestors. Analysis of complete genomes of 3 sylvatic and 4 human strains revealed high conservation of both the 5?- and 3?-untranslated
regions but considerable divergence within the open reading frame. Additionally, the two ecotypes did not differ significantly in replication dynamics in cultured human liver (Huh-7), monkey kidney (Vero) or mosquito (C6/36) cells, although significant inter-strain variation within ecotypes was detected. These findings are in partial agreement with previous studies of DENV-2, where human strains produced a larger number of progeny than sylvatic strains in human liver cells but not in monkey or mosquito cells.


Physical Location

LocationCall NumberAvailable FormatAvailability
Philippine Council for Health Research and Development Library Fulltext pdf